The dilution calculator equation. The Tocris dilution calculator is based on the following equation: Concentration (start) x Volume (start) = Concentration (final) x Volume (final) This equation is commonly abbreviated as: C 1 V 1 = C 2 V 2. An example of a dilution calculation using the Tocris dilution calculator. Although not a serial dilution, the below is an example of a two-fold dilution. Problem #4: To make a two-fold dilution of 10 mL of solution, what amount of solvent would you use and how would you do this? To perform a dilution you can always use the equation: (Volume of solution)/(vol of solution + vol of solvent).
Review of Dilution, Concentration, and Stock Solutions
A dilution is a solution made by adding more solvent to a more concentrated solution (stock solution), which reduces the concentration of the solute. An example of a dilute solution is tap water, which is mostly water (solvent), with a small amount of dissolved minerals and gasses (solutes).
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An example of a concentrated solution is 98 percent sulfuric acid (~18 M). The primary reason you start with a concentrated solution and then dilute it to make a dilution is that it's very difficult—and sometimes impossible—to accurately measure solute to prepare a dilute solution, so there would be a large degree of error in the concentration value.
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Use the law of conservation of mass to perform the calculation for the dilution:
MdilutionVdilution = MstockVstock
Dilution Example
Microbiology Serial Dilution Examples
As an example, say you need to prepare 50 milliliters of a 1.0 M solution from a 2.0 M stock solution. Your first step is to calculate the volume of stock solution that is required.
Serial Dilution Equation Examples
MdilutionVdilution = MstockVstock (1.0 M)(50 ml) = (2.0 M)(x ml) x = [(1.0 M)(50 ml)]/2.0 M x = 25 ml of stock solution
Serial Dilution Calculation Examples For Research
To make your solution, pour 25 ml of stock solution into a 50 ml volumetric flask. Dilute it with solvent to the 50 ml line.
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Avoid This Common Dilution Mistake
Microbiology Dilution Problems With Answe…
It's a common mistake to add too much solvent when making the dilution. Make sure you pour the concentrated solution into the flask and then dilute it to the volume mark. Do not, for example, mix 250 ml of concentrated solution with 1 liter of solvent to make a 1-liter solution.